›› 2011, Vol. 23 ›› Issue (6): 406-409.doi: 10.3969/j.issn.1004-616x.2011.06.001

• 核辐射安全专家论坛 • Previous Articles     Next Articles

Homologous recombination repair gene RAD51 could increase the genomic stability of PTEN deleted cells

CHEN Zhong-min，XU Qin-zhi，HU Ying-chun，HUO Yan-ying，ZHOU Ping-kun，WU De-chang   

  1. 1. Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850; 2. Center for Disease Control And Prevention, Second Artillery Force of PLA, Beijing 100071, China
  • Received:2011-09-19 Revised:2011-10-20 Online:2011-11-30 Published:2011-11-30
  • Contact: XU Qin-zhi

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Abstract: OBJECTIVE: To explore the effect of genomic stability of PTEN-deficient mice embryonic fibroblasts after transfection with RAD51，an important homologous recombination repair gene. METHODS： Immunofluorescence assay was used to observe the number of spontaneous γ-H2AX foci. Neutral single cell gel electrophoresis was applied to detect spontaneous and radiation-induced DNA double-strand breaks in both PTEN-deficient and PTEN wild-type cells. RESULTS：PTEN deletion led to an increase of spontaneous γ-H2AX foci and the length of tail moment， indicating DNA double-strand breaks. Transfection with RAD51 increased cell survival and decreased radiation-induced DNA double-strand breaks of PTEN-deficient cells. CONCLUSION：Homologous recombination gene RAD51 could increase genomic stability of PTEN-deficient cells.

Key words: PTEN, RAD51, genomic stability